Plant Microtechnique and Microscopy by Steven E. Ruzin, , available at Book Depository with free delivery worldwide. Plant Microtechnique BOT Course Objectives: Managing the techniques of microscopic slides making, microscopic measurements and methods of. Plant microtechnique has generated renewed interest in recent years, due in part to the need for molecular biologists to visualize a gene or gene product in the.
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PDF | On Jul 7, , N. J. Chaffey and others published Plant microtechnique and microscopyBy Plant microtechnique and microscopy. hybridization. Keep in mind that these are just a few of the myriad useful techniques found in the old literature. TISSUE CLEARING. Plant cells and tissues . REVIEWS New Phytol. (), , 57– Books. Microtechnique. Plant microtechnique and microscopy. By Steven E. Ruzin. xij pages.
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Old Password. New Password. The traditional paraffin method is covered in explicit step-by-step protocols, with theoretical background included to give students the tools required to design their own experiments and interpret existing results.
In addition, the theory and practice of modern applications, such as methacrylate embedding and sectioning, microwave tissue processing, flourescence histochemistry, and in situ hybridization, are documented thoroughly.
Preceding Chapter One are ten single-page "Quick Start" protocols.
Patterned after computer software manuals, these abbreviated instructions provide the experienced student with the fundamental steps to complete the ten microtechnique protocols most frequently used today. Appendices on laboratory practice chemical toxicites, common calculations, and buffer tables are provided to make this volume a valuable addition to every biological laboratory.
Plant Microtechnique and Microscopy contains a definitive chapter on microscopy and, with diagrams and text, describes the optical principles of techniques such as phase contrast, DIC, confocal and deconvolution wide-field microscopy. Also included is an extensive appendix on optics and its application to the microscope.
The book's bibliography of over references is a substantial resource for any student of histology, histological technique, or microscopy.
Table of contents 1. Microscopy ; History of the compound microscope ; Principles of specimen illumination ; 3.
Chemical Fixation of Tissues ; The quality of fixation ; The mechanics of fixation ; Coagulating Fixatives ; Crosslinking Agents ; Other fixation methods ; Recommendations for fixing plant tissues ; 4.
Tissue Dehydration ; General protocol ; Dehydration using a graded dehydration series ; Dehydration using chemical agents ; 5.
Sectioning and Mounting ; Mounting blocks to microtome stubs ; The quality of sectioning ; The microtome knife ; Setting up the microtome ; Sectioning Methods ; Mounting sections to glass slides ; 7. Staining ; Dye Chemistry ; Staining equipment ; General histological staining ; Other microtechniques stains and protocols ; Mounting the coverslip ; Coverslip mounting media ; 8.
Special Methods ; Clearing tissues with NaOH and chloral hydrate ; Clearing tissues without removing cytoplasmic components ; Macerating woody tissues ; Macerating non-woody tissues ; Bleaching tissues ; Preserving color in whole mounted specimens ; Histochemistry and Cytochemistry ; The relationship of the probe to the target ; Cytochemical localization of cell components ; Cytochemical localization of enzymes ; Cytochemical Fluorescence Microscopy ; Review quote "I am grateful to Steve Ruzin for assembling this incredibly useful collection of information in such a convenient and handy form.